Structural highlights
6chg is a 7 chain structure with sequence from Candida sphaerica. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , |
| Gene: | KLLA0_E24487g (Candida sphaerica), KLLA0_C10945g (Candida sphaerica), SET1, KLLA0F24134g (Candida sphaerica), KLLA0_A08800g (Candida sphaerica), KLLA0_E03521g (Candida sphaerica) |
| Activity: | Histone-lysine N-methyltransferase, with EC number 2.1.1.43 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[SET1_KLULA] Catalytic component of the COMPASS (Set1C) complex that specifically mono-, di- and trimethylates histone H3 to form H3K4me1/2/3, which subsequently plays a role in telomere length maintenance and transcription elongation regulation.
Publication Abstract from PubMed
The SET1/MLL family of histone methyltransferases is conserved in eukaryotes and regulates transcription by catalyzing histone H3K4 mono-, di-, and tri-methylation. These enzymes form a common five-subunit catalytic core whose assembly is critical for their basal and regulated enzymatic activities through unknown mechanisms. Here, we present the crystal structure of the intact yeast COMPASS histone methyltransferase catalytic module consisting of Swd1, Swd3, Bre2, Sdc1, and Set1. The complex is organized by Swd1, whose conserved C-terminal tail not only nucleates Swd3 and a Bre2-Sdc1 subcomplex, but also joins Set1 to construct a regulatory pocket next to the catalytic site. This inter-subunit pocket is targeted by a previously unrecognized enzyme-modulating motif in Swd3 and features a doorstop-style mechanism dictating substrate selectivity among SET1/MLL family members. By spatially mapping the functional components of COMPASS, our results provide a structural framework for understanding the multifaceted functions and regulation of the H3K4 methyltransferase family.
Crystal Structure of the COMPASS H3K4 Methyltransferase Catalytic Module.,Hsu PL, Li H, Lau HT, Leonen C, Dhall A, Ong SE, Chatterjee C, Zheng N Cell. 2018 Aug 23;174(5):1106-1116.e9. doi: 10.1016/j.cell.2018.06.038. Epub 2018, Aug 9. PMID:30100181[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hsu PL, Li H, Lau HT, Leonen C, Dhall A, Ong SE, Chatterjee C, Zheng N. Crystal Structure of the COMPASS H3K4 Methyltransferase Catalytic Module. Cell. 2018 Aug 23;174(5):1106-1116.e9. doi: 10.1016/j.cell.2018.06.038. Epub 2018, Aug 9. PMID:30100181 doi:http://dx.doi.org/10.1016/j.cell.2018.06.038
