Structural highlights
Publication Abstract from PubMed
Lytic polysaccharide monooxygenases (LPMOs) are industrial enzymes which are gaining use in second generation bioethanol production from lignocellulose by acting in synergy with glycoside hydrolases. Here we present the X-ray crystal structure of an AA9 fungal LPMO from Aspergillus fumigatus and a variant which has been shown to have better performance at elevated temperatures. Based on the structures, thermal denaturation data and theoretical calculations, we provide a suggestion for the structural basis of the improved stability.
Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant.,Lo Leggio L, Weihe CD, Poulsen JN, Sweeney M, Rasmussen F, Lin J, De Maria L, Wogulis M Carbohydr Res. 2018 Nov;469:55-59. doi: 10.1016/j.carres.2018.08.009. Epub 2018, Aug 14. PMID:30296642[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Lo Leggio L, Weihe CD, Poulsen JN, Sweeney M, Rasmussen F, Lin J, De Maria L, Wogulis M. Structure of a lytic polysaccharide monooxygenase from Aspergillus fumigatus and an engineered thermostable variant. Carbohydr Res. 2018 Nov;469:55-59. doi: 10.1016/j.carres.2018.08.009. Epub 2018, Aug 14. PMID:30296642 doi:http://dx.doi.org/10.1016/j.carres.2018.08.009
