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User:Wayne Decatur/Sandbox 1 vs 21 vs 2 vs 12
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1 vs 21 vs 2 vs 12
The Alignment
Alignment of yeast Snm1p to protions of other yeast proteins and Pyrococcus horikoshii Rpp21p: Snm1 MNKDQAEKYQERSLRQKYNLLHVLP-------TLNSRALSGLYYKNFHNS-VKRYQIMLP 1x0t.1.A -----------ERIDTLFTLAERV--------ARYSPDLAKRYVELALEI-QKKAKVKIP Rpr2 -------------LNYLYQISAYQTRARQKARTDAHTPLA-RNYIKSMDLISKKTKTSLL Rpa12 ------------------------------------------------------------ Snm1 EQLKSGKFCSHCGCVYVPNFNASLQLTTNTEQGDSDELGGESMEGPKKCIQVNCLNCEKS 1x0t.1.A RKWK-RRYCKRCHTFLIPGVNARVRLRTKR----------------MPHVVITCLECGYI Rpr2 PTIK-RTICKKCHRLLWTPKKLEITSD--------------------GALSVMC-GCGTV Rpa12 -----KEKCPQCGNEEM------NYHTLQ--LRSADE---------GATVFYTCTSCGYK Snm1 KLFEWKSEFVVPTFGQDVSPMINSTSSGKVSYAVKKPQKSKTSTGKERSKKRKLNSLTNL 1x0t.1.A MRYPYLREVK-------------------------------------------------- Rpr2 KRFNIGADPNYRTYSEREGNLLNS------------------------------------ Rpa12 FRTNN------------------------------------------------------- Snm1 LSKRNQEKKMEKKKSSSLSLESFMKS 1x0t.1.A -------------------------- Rpr2 -------------------------- Rpa12 --------------------------
(Specifically, QKRKKATREVKQKRKKAT is left off C-terminus of 1xOt chain A (Rpp21p); MGKKAHGGKMKPEIDENGTLLVPPPRTIANQDHFHR is left off N-terminus of Rpr2p; just C-terminus of Rpa12p is shown; all residues of Snm1 are represented. All sequences from S. cerevisiae except 1x0t, which is Pyrococcus horikoshii.)
Structures superposed
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Understanding Rpr2p in the Yeast RNase P structure
To learn more about Rpr2p in the yeast RNase P cryo-EM structure [1] go to here and take the tour in sections 'Rpr2p overview' and 'Rpr2p binds zinc'. (Note that it is a work-in-progress.)
For smaller screens, such as tablets, use here
References
- ↑ Lan P, Tan M, Zhang Y, Niu S, Chen J, Shi S, Qiu S, Wang X, Peng X, Cai G, Cheng H, Wu J, Li G, Lei M. Structural insight into precursor tRNA processing by yeast ribonuclease P. Science. 2018 Sep 27. pii: science.aat6678. doi: 10.1126/science.aat6678. PMID:30262633 doi:http://dx.doi.org/10.1126/science.aat6678
