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8drh
From Proteopedia
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| Ligands: | , , , , , , , | ||||||
| Related: | 8PSH
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
HIGH RESOLUTION NMR STRUCTURE OF THE D(GCGTCAGG)R(CCUGACGC) HYBRID, MINIMIZED AVERAGE STRUCTURE
Overview
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-Rp)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
About this Structure
8DRH is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
Structure of a stereoregular phosphorothioate DNA/RNA duplex., Bachelin M, Hessler G, Kurz G, Hacia JG, Dervan PB, Kessler H, Nat Struct Biol. 1998 Apr;5(4):271-6. PMID:9546216
Page seeded by OCA on Mon Mar 31 05:45:05 2008
