3bsn

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spinqualitylabelsall models PDB ID 3bsn Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image
, resolution 1.80Å
Sites:	, , , , , , and
Ligands:	, , , , , ,
Activity:	RNA-directed RNA polymerase, with EC number 2.7.7.48
Domains:	RNA_dep_RNAP
Related:	1SH0, 3BSO
Resources:	FirstGlance, OCA, PDBsum, RCSB
Coordinates:	save as pdb, mmCIF, xml

Norwalk Virus polymerase bound to 5-nitrocytidine triphosphate and primer-template RNA

Overview

Crystal structures of Norwalk virus polymerase bound to an RNA primer-template duplex and either the natural substrate CTP or the inhibitor 5-nitrocytidine triphosphate have been determined to 1.8A resolution. These structures reveal a closed conformation of the polymerase that differs significantly from previously determined open structures of calicivirus and picornavirus polymerases. These closed complexes are trapped immediately prior to the nucleotidyl transfer reaction, with the triphosphate group of the nucleotide bound to two manganese ions at the active site, poised for reaction to the 3'-hydroxyl group of the RNA primer. The positioning of the 5-nitrocytidine triphosphate nitro group between the alpha-phosphate and the 3'-hydroxyl group of the primer suggests a novel, general approach for the design of antiviral compounds mimicking natural nucleosides and nucleotides.

About this Structure

3BSN is a Protein complex structure of sequences from Norwalk virus. Full crystallographic information is available from OCA.

Reference

Structural insights into mechanisms of catalysis and inhibition in norwalk virus polymerase., Zamyatkin DF, Parra F, Alonso JM, Harki DA, Peterson BR, Grochulski P, Ng KK, J Biol Chem. 2008 Mar 21;283(12):7705-12. Epub 2008 Jan 9. PMID:18184655

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