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2jx2
From Proteopedia
Solution conformation of RNA-bound NELF-E RRM
Overview
The E subunit of the human heterotetrameric negative transcription elongation factor (NELF-E) contains a canonical betaalphabetabetaalphabeta RNA recognition motif (RRM) that binds to a wide variety of RNA sequences. These induce very similar conformational changes in the RRM as determined by nuclear magnetic resonance spectroscopy. Although the RNA binding interface of a canonical RRM is mainly located at its beta-sheet surface, for NELF-E RRM large chemical shift perturbations are observed for residues in the flexible C-terminal region and the loop between beta 3 and alpha 2, and both regions are distant from the interface. We determined the solution structure of single-stranded transactivator responsive element (TAR) RNA-bound NELF-E RRM. This structure clearly shows that RNA binding to NELF-E RRM induces formation of a helix in the C-terminus. The RNA-bound form of NELF-E RRM is very similar to the RNA-bound form of U1A RRM, although the C-terminus of the NELF-E RRM is unstructured in the free protein, whereas it is helical in the U1A protein. Thus, RNA binding to NELF-E RRM induces a conformational change toward the U1A structure, resulting in highly similar RNA binding conformations for both proteins.
About this Structure
2JX2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
NELF-E RRM Undergoes Major Structural Changes in Flexible Protein Regions on Target RNA Binding., Rao JN, Schweimer K, Wenzel S, Wohrl BM, Rosch P, Biochemistry. 2008 Mar 25;47(12):3756-61. Epub 2008 Feb 28. PMID:18303858
