User:Nicholas Bantz/Sandbox 1
From Proteopedia
Human lysine-specific histone demethylase (LSD-l))
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Introduction LSD-1 is a lysine demethylase. A histone is a blah blah blah and can be seen in Fig. 1. Structure Tower Domain The is a protrusion off the main protein body of LSD-1 comprised of 100 residues, which form 2 π-helices. The longer helix, TπA, is an LSD-1 specific element that has not been found in any other oxidase proteins. The shorter helix, TπB, is very near to the active site of the oxidase domain. In fact, TπB connects directly to helix πD of the oxidase domain through a highly conserved connector loop. The exact function of the tower domain is not known, but it is proposed to regulate the size of the active site chamber through this . The TπB-πD interaction is responsible for the proper positioning of Phe538, a side chain of πD that is located in the catalytic chamber. In addition, the TπB-πD interaction positions πD in the correct manner to provide hydrogen bonding to Tyr761. Tyr761 is positioned in the catalytic chamber very close to the FAD cofactor, and aids in the binding of the lysine substrate. Therefore, the base of the tower domain forms a direct connection to the oxidase domain and plays a crucial role in the shape and catalytic activity of the active site. In fact, removing the tower domain via a mutation resulted in a drastic decrease in catalytic efficiency.(1) The tower domain has also been found to interact with other proteins and complexes, such as CoREST (see Figure 2), as a molecular lever to allosterically regulate the catalytic activity of the active site. (insert other source here) Overall, the exact function of the tower domain has not yet been determined, but it is known to be vital to the catalytic activity of LSD-1. SWIRM DomainThe domain is a blah blah blah blah. These are the Oxidase DomainThe is ALSO a blah blah blah. The is vitally important because... FAD CofactorThe is ALSO a blah blah blah. Mechanism of Active SiteThis is a picture of the . These are the different Medical ImplicationsReferences
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Student Contributors
- Nicholas Bantz
- Cody Carley
- Michael Thomas
