| Structural highlights
6g04 is a 2 chain structure with sequence from Baker's yeast. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Related: | 6g03 |
| Gene: | TSR2, YLR435W (Baker's yeast), RPS26A, RPS26, YGL189C, G1355 (Baker's yeast) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[TSR2_YEAST] Required for 20S pre-rRNA processing.[1]
Publication Abstract from PubMed
Disordered extensions at the termini and short internal insertions distinguish eukaryotic ribosomal proteins (r-proteins) from their anucleated archaeal counterparts. Here, we report an NMR structure of such a eukaryotic-specific segment (ESS) in the r-protein eS26 in complex with the escortin Tsr2. The structure reveals how ESS attracts Tsr2 specifically to importin:eS26 complexes entering the nucleus in order to trigger non-canonical RanGTP-independent disassembly. Tsr2 then sequesters the released eS26 and prevents rebinding to the importin, providing an alternative allosteric mechanism to terminate the process of nuclear import. Notably, a Diamond-Blackfan anemia-associated Tsr2 mutant protein is impaired in binding to ESS, unveiling a critical role for this interaction in human hematopoiesis. We propose that eS26-ESS and Tsr2 are components of a nuclear sorting system that co-evolved with the emergence of the nucleocytoplasmic barrier and transport carriers.
Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2.,Schutz S, Michel E, Damberger FF, Oplova M, Pena C, Leitner A, Aebersold R, Allain FH, Panse VG Nat Commun. 2018 Sep 10;9(1):3669. doi: 10.1038/s41467-018-06160-x. PMID:30201955[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Peng WT, Robinson MD, Mnaimneh S, Krogan NJ, Cagney G, Morris Q, Davierwala AP, Grigull J, Yang X, Zhang W, Mitsakakis N, Ryan OW, Datta N, Jojic V, Pal C, Canadien V, Richards D, Beattie B, Wu LF, Altschuler SJ, Roweis S, Frey BJ, Emili A, Greenblatt JF, Hughes TR. A panoramic view of yeast noncoding RNA processing. Cell. 2003 Jun 27;113(7):919-33. PMID:12837249
- ↑ Schutz S, Michel E, Damberger FF, Oplova M, Pena C, Leitner A, Aebersold R, Allain FH, Panse VG. Molecular basis for disassembly of an importin:ribosomal protein complex by the escortin Tsr2. Nat Commun. 2018 Sep 10;9(1):3669. doi: 10.1038/s41467-018-06160-x. PMID:30201955 doi:http://dx.doi.org/10.1038/s41467-018-06160-x
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