Structural highlights
Publication Abstract from PubMed
Bacterial cytochrome P450s form a remarkable clade of the P450 superfamily of oxidative hemoproteins, and are often involved in the biosynthesis of complex natural products. Those in a subgroup known as "Oxy enzymes" play a crucial role in the biosynthesis of glycopeptide antibiotics, including vancomycin and teicoplanin. The Oxy enzymes catalyze crosslinking of aromatic residues in the non-ribosomal antibiotic precursor peptide while it remains bound to the non-ribosomal peptide synthetase (NRPS); this crosslinking secures the three-dimensional structure of the glycopeptide, crucial for antibiotic activity. We have characterized OxyBtei , the first of the Oxy enzymes in teicoplanin biosynthesis. Our results reveal that OxyBtei possesses a structure similar to those of other Oxy proteins and is active in crosslinking NRPS-bound peptide substrates. However, OxyBtei displays a significantly altered activity spectrum against peptide substrates compared to its well-studied vancomycin homologue.
Cytochrome P450 OxyB Catalyzes the First Phenolic Coupling Step in Teicoplanin Biosynthesis.,Haslinger K, Maximowitsch E, Brieke C, Koch A, Cryle MJ Chembiochem. 2014 Oct 30. doi: 10.1002/cbic.201402441. PMID:25358800[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Haslinger K, Maximowitsch E, Brieke C, Koch A, Cryle MJ. Cytochrome P450 OxyB Catalyzes the First Phenolic Coupling Step in Teicoplanin Biosynthesis. Chembiochem. 2014 Oct 30. doi: 10.1002/cbic.201402441. PMID:25358800 doi:http://dx.doi.org/10.1002/cbic.201402441
