| Structural highlights
6mnz is a 2 chain structure with sequence from Aciba. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Ligands: | , |
| Gene: | ribB, ribBA, A7M79_16415, A7M90_07480, A7N09_07660, AB895_1395, APD06_16235, AZE33_19550, B4R90_18035, BGC29_12000, BWP00_07280, C2U32_13770, CAS83_08960, CBE85_14000, CHQ89_12900, CPI82_04655, IX87_13995, LV38_00273 (ACIBA) |
| Activity: | 3,4-dihydroxy-2-butanone-4-phosphate synthase, with EC number 4.1.99.12 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[V5V8R9_ACIBA] Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.[HAMAP-Rule:MF_00180][SAAS:SAAS00183795]
Publication Abstract from PubMed
Calprotectin (CP) inhibits bacterial viability through extracellular chelation of transition metals. However, how CP influences general metabolism remains largely unexplored. We show here that CP restricts bioavailable Zn and Fe to the pathogen Acinetobacter baumannii, inducing an extensive multi-metal perturbation of cellular physiology. Proteomics reveals severe metal starvation, and a strain lacking the candidate Zn(II) metallochaperone ZigA possesses altered cellular abundance of multiple essential Zn-dependent enzymes and enzymes in de novo flavin biosynthesis. The DeltazigA strain exhibits decreased cellular flavin levels during metal starvation. Flavin mononucleotide provides regulation of this biosynthesis pathway, via a 3,4-dihydroxy-2-butanone 4-phosphate synthase (RibB) fusion protein, RibBX, and authentic RibB. We propose that RibBX ensures flavin sufficiency under CP-induced Fe limitation, allowing flavodoxins to substitute for Fe-ferredoxins as cell reductants. These studies elucidate adaptation to nutritional immunity and define an intersection between metallostasis and cellular metabolism in A. baumannii.
Multi-metal Restriction by Calprotectin Impacts De Novo Flavin Biosynthesis in Acinetobacter baumannii.,Wang J, Lonergan ZR, Gonzalez-Gutierrez G, Nairn BL, Maxwell CN, Zhang Y, Andreini C, Karty JA, Chazin WJ, Trinidad JC, Skaar EP, Giedroc DP Cell Chem Biol. 2019 Mar 15. pii: S2451-9456(19)30066-2. doi:, 10.1016/j.chembiol.2019.02.011. PMID:30905682[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang J, Lonergan ZR, Gonzalez-Gutierrez G, Nairn BL, Maxwell CN, Zhang Y, Andreini C, Karty JA, Chazin WJ, Trinidad JC, Skaar EP, Giedroc DP. Multi-metal Restriction by Calprotectin Impacts De Novo Flavin Biosynthesis in Acinetobacter baumannii. Cell Chem Biol. 2019 Mar 15. pii: S2451-9456(19)30066-2. doi:, 10.1016/j.chembiol.2019.02.011. PMID:30905682 doi:http://dx.doi.org/10.1016/j.chembiol.2019.02.011
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