Structural highlights
Publication Abstract from PubMed
Modular self-assembly of biomolecules in two dimensions (2D) is straightforward with DNA but has been difficult to realize with proteins, due to the lack of modular specificity similar to Watson-Crick base pairing. Here we describe a general approach to design 2D arrays using de novo designed pseudosymmetric protein building blocks. A homodimeric helical bundle was reconnected into a monomeric building block, and the surface was redesigned in Rosetta to enable self-assembly into a 2D array in the C12 layer symmetry group. Two out of ten designed arrays assembled to micrometer scale under negative stain electron microscopy, and displayed the designed lattice geometry with assembly size up to 100 nm under atomic force microscopy. The design of 2D arrays with pseudosymmetric building blocks is an important step toward the design of programmable protein self-assembly via pseudosymmetric patterning of orthogonal binding interfaces.
Self-Assembling 2D Arrays with de Novo Protein Building Blocks.,Chen Z, Johnson MC, Chen J, Bick MJ, Boyken SE, Lin B, De Yoreo JJ, Kollman JM, Baker D, DiMaio F J Am Chem Soc. 2019 May 10. doi: 10.1021/jacs.9b01978. PMID:31050411[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Chen Z, Johnson MC, Chen J, Bick MJ, Boyken SE, Lin B, De Yoreo JJ, Kollman JM, Baker D, DiMaio F. Self-Assembling 2D Arrays with de Novo Protein Building Blocks. J Am Chem Soc. 2019 May 10. doi: 10.1021/jacs.9b01978. PMID:31050411 doi:http://dx.doi.org/10.1021/jacs.9b01978
