Structural highlights
Function
[ACOD_HUMAN] Terminal component of the liver microsomal stearyl-CoA desaturase system, that utilizes O(2) and electrons from reduced cytochrome b5 to catalyze the insertion of a double bond into a spectrum of fatty acyl-CoA substrates including palmitoyl-CoA and stearoyl-CoA.
Publication Abstract from PubMed
Stearoyl-coenzyme A desaturase-1 (SCD1) has an important role in lipid metabolism, and SCD1 inhibitors are potential therapeutic agents for the treatment of metabolic diseases and cancers. Here we report the 3.25-A crystal structure of human SCD1 in complex with its substrate, stearoyl-coenzyme A, which defines the new SCD1 dimetal catalytic center and reveals the determinants of substrate binding to provide insights into the catalytic mechanism of desaturation of the stearoyl moiety. The structure also provides a mechanism for localization of SCD1 in the endoplasmic reticulum: human SCD1 folds around a tight hydrophobic core formed from four long alpha-helices that presumably function as an anchor spanning the endoplasmic reticulum membrane. Furthermore, our results provide a framework for the rational design of pharmacological inhibitors targeting the SCD1 enzyme.
Crystal structure of human stearoyl-coenzyme A desaturase in complex with substrate.,Wang H, Klein MG, Zou H, Lane W, Snell G, Levin I, Li K, Sang BC Nat Struct Mol Biol. 2015 Jul;22(7):581-5. doi: 10.1038/nsmb.3049. Epub 2015 Jun , 22. PMID:26098317[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Wang H, Klein MG, Zou H, Lane W, Snell G, Levin I, Li K, Sang BC. Crystal structure of human stearoyl-coenzyme A desaturase in complex with substrate. Nat Struct Mol Biol. 2015 Jul;22(7):581-5. doi: 10.1038/nsmb.3049. Epub 2015 Jun , 22. PMID:26098317 doi:http://dx.doi.org/10.1038/nsmb.3049
