This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
3brd
From Proteopedia
CSL (Lag-1) bound to DNA with Lin-12 RAM peptide, P212121
Overview
The Notch pathway is a conserved cell-to-cell signaling mechanism, in which extracellular signals are transduced into transcriptional outputs through the nuclear effector CSL. CSL is converted from a repressor to an activator through the formation of the CSL-NotchIC-Mastermind ternary complex. The RAM domain of NotchIC avidly interacts with CSL; however, its role in assembly of the CSL-NotchIC-Mastermind ternary complex is not understood. Here we provide a comprehensive thermodynamic, structural, and biochemical analysis of the RAM-CSL interaction for components from both mouse and worm. Our binding data shows that RAM and CSL form a high affinity complex in the presence or absence of DNA. Our structural studies reveal a striking distal conformational change in CSL upon RAM binding, which creates a docking site for Mastermind to bind to the complex. Finally, we show that the addition of a RAM peptide in trans facilitates formation of the CSL-NotchIC-Mastermind ternary complex in vitro.
About this Structure
3BRD is a Protein complex structure of sequences from Caenorhabditis elegans. Full crystallographic information is available from OCA.
Reference
RAM induced allostery facilitates assembly of a notch pathway active transcription complex., Friedmann DR, Wilson JJ, Kovall RA, J Biol Chem. 2008 Apr 1;. PMID:18381292 Page seeded by OCA on Thu Apr 24 09:33:18 2008
Categories: Caenorhabditis elegans | Protein complex | Kovall, R A. | Wilson, J J. | Ank repeat | Developmental protein | Differentiation | Dna binding protein/dna complex | Dna-binding | Egf-like domain | Glycoprotein | Membrane | Notch | Protein-dna complex | Signaling | Transcription | Transmembrane
