Structural highlights
Function
[TIP20_YEAST] Required for protein transport between the Golgi and the endoplasmic reticulum. May contribute to tethering of coatomer-coated retrograde transport vesicles to the ER membrane through interaction with and stabilization of the SNARE complex.[1] [2]
Publication Abstract from PubMed
Multisubunit tethering complexes are essential for intracellular trafficking and have been proposed to mediate the initial interaction between vesicles and the membranes with which they fuse. Here we report initial structural characterization of the Dsl1p complex, whose three subunits are essential for trafficking from the Golgi apparatus to the endoplasmic reticulum (ER). Crystal structures reveal that two of the three subunits, Tip20p and Dsl1p, resemble known subunits of the exocyst complex, establishing a structural connection among several multisubunit tethering complexes and implying that many of their subunits are derived from a common progenitor. We show, moreover, that Tip20p and Dsl1p interact directly via N-terminal alpha-helices. Finally, we establish that different Dsl1p complex subunits bind independently to different ER SNARE proteins. Our results map out two alternative protein-interaction networks capable of tethering COPI-coated vesicles, via the Dsl1p complex, to ER membranes.
Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex.,Tripathi A, Ren Y, Jeffrey PD, Hughson FM Nat Struct Mol Biol. 2009 Feb;16(2):114-23. Epub 2009 Jan 18. PMID:19151722[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kraynack BA, Chan A, Rosenthal E, Essid M, Umansky B, Waters MG, Schmitt HD. Dsl1p, Tip20p, and the novel Dsl3(Sec39) protein are required for the stability of the Q/t-SNARE complex at the endoplasmic reticulum in yeast. Mol Biol Cell. 2005 Sep;16(9):3963-77. Epub 2005 Jun 15. PMID:15958492 doi:E05-01-0056
- ↑ Sweet DJ, Pelham HR. The TIP1 gene of Saccharomyces cerevisiae encodes an 80 kDa cytoplasmic protein that interacts with the cytoplasmic domain of Sec20p. EMBO J. 1993 Jul;12(7):2831-40. PMID:8334998
- ↑ Tripathi A, Ren Y, Jeffrey PD, Hughson FM. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nat Struct Mol Biol. 2009 Feb;16(2):114-23. Epub 2009 Jan 18. PMID:19151722 doi:10.1038/nsmb.1548
