Structural highlights
Function
[LACI_ECOLI] Repressor of the lactose operon. Binds allolactose as an inducer.
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
Crystal structures of the Lac repressor, with and without isopropyithiogalactoside (IPTG), and the repressor bound to operator have provided a model for how the binding of the inducer reduces the affinity of the repressor for the operator. However, because of the low resolution of the operator-bound structure (4.8 A), the model for the allosteric transition was presented in terms of structural elements rather than in terms of side chain interactions. Here we have constructed a dimeric Lac repressor and determined its structure at 2.6 A resolution in complex with a symmetric operator and the anti-inducer orthonitrophenylfucoside (ONPF). The structure enables the induced (IPTG-bound) and repressed (operator-bound) conformations of the repressor to be compared in atomic detail. An extensive network of interactions between the DNA-binding and core domains of the repressor suggests a possible mechanism for the allosteric transition.
A closer view of the conformation of the Lac repressor bound to operator.,Bell CE, Lewis M Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:10700279[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Bell CE, Lewis M. A closer view of the conformation of the Lac repressor bound to operator. Nat Struct Biol. 2000 Mar;7(3):209-14. PMID:10700279 doi:10.1038/73317
