1uvk
From Proteopedia
THE STRUCTURAL BASIS FOR RNA SPECIFICITY AND CA2 INHIBITION OF AN RNA-DEPENDENT RNA POLYMERASE PHI6P2 DEAD-END COMPLEX
Overview
The RNA-dependent RNA polymerase of bacteriophage phi6 transcribes mRNA from the three segments of the dsRNA viral genome. We have cocrystallized RNA oligonucleotides with the polymerase, revealing the mode of binding of RNA templates. This binding is somewhat different from that previously seen for DNA oligomers, leading to additional RNA-protein hydrogen bonds, consistent with a preference for RNA. Activation of the RNA/polymerase complex by the addition of substrate and Mg2+ initiates a single round of reaction within the crystal to form a dead-end complex that partially collapses within the enzyme active site. By replacing Mg2+ with Ca2+, we have been able to capture the inhibited complex which shows distortion that explains the structural basis for the inhibition of such polymerases by Ca2+.
About this Structure
1UVK is a Protein complex structure of sequences from Pseudomonas phage phi6. Full crystallographic information is available from OCA.
Reference
The structural basis for RNA specificity and Ca2+ inhibition of an RNA-dependent RNA polymerase., Salgado PS, Makeyev EV, Butcher SJ, Bamford DH, Stuart DI, Grimes JM, Structure. 2004 Feb;12(2):307-16. PMID:14962391 Page seeded by OCA on Wed Apr 30 13:56:23 2008
