6l8u

Structural highlights

Function

[BN3D2_HUMAN] O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA, acting as a capping enzyme (PubMed:28119416). Less efficiently, also methylates the 5' monophosphate of pre-miRNAs, acting as a negative regulator of miRNA processing (PubMed:23063121, PubMed:28119416). The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Able to mediate methylation of pre-miR-145, as well as other pre-miRNAs (PubMed:23063121). There is some controversy about the methylation of pre-miR-145, since the dimethylation first described as the specific enzymatic activity cannot be reproduced by a more recent work which observes a monomehtylation of pre-miR-145 but two orders weaker than the methylation of cytosolic histidyl tRNA (PubMed:23063121, PubMed:28119416, PubMed:30127802).^{[1] [2] [3]}

References

1. ↑ Xhemalce B, Robson SC, Kouzarides T. Human RNA methyltransferase BCDIN3D regulates microRNA processing. Cell. 2012 Oct 12;151(2):278-88. doi: 10.1016/j.cell.2012.08.041. PMID:23063121 doi:http://dx.doi.org/10.1016/j.cell.2012.08.041
2. ↑ Martinez A, Yamashita S, Nagaike T, Sakaguchi Y, Suzuki T, Tomita K. Human BCDIN3D monomethylates cytoplasmic histidine transfer RNA. Nucleic Acids Res. 2017 May 19;45(9):5423-5436. doi: 10.1093/nar/gkx051. PMID:28119416 doi:http://dx.doi.org/10.1093/nar/gkx051
3. ↑ Tomita K, Liu Y. Human BCDIN3D Is a Cytoplasmic tRNA(His)-Specific 5'-Monophosphate Methyltransferase. Front Genet. 2018 Aug 3;9:305. doi: 10.3389/fgene.2018.00305. eCollection 2018. PMID:30127802 doi:http://dx.doi.org/10.3389/fgene.2018.00305