Function
The Glucose-6-Phosphate Dehydrogenase is involved in the processing of carbohydrates as it has important roles in the glucose metabolic process (glycolysis and pentose phosphate pathway).
It also has a role in protecting cells from destruction as it produces the co-factor NADPH which plays a role in protecting cells from reactive oxygen species [1].
Genomic context
It is coded by the G6PD gene (1461 nucleotides).
Catalytic activity
D-glucose 6-phosphate + NAD+ → 6-phospho-D-glucono-1,5-lactone + H+ + NADH[2]
KM=114 µM for G6PD (with NADP), KM=69 µM for G6PD (with NAD),
KM=8.0 µM for NADP, KM=160 µM for NAD.
Its regulation depends on the concentration of substrate and coenzyme, rate limiting step in pentose phosphate pathway[3].
Optimal activity conditions
Optimum pH is 5.4 - 8.9.
Evolutionary conservation
The different structures conserved evolutionary can be observed according to the scale following.
Check, as determined by ConSurfDB.
Mutations
Mutagenesis inducing catalytic activity loss: +200 mutations have been identified. A change of amino acids leads to disruption of the normal
structure/function/reduce the expression of enzymes.
Structural highlights
It is formed of a homodimer, so a dimer of two identical monomers[4]. Each monomer is composed of 2 domains,
Depending on several conditions, it can dimerize to form tetramers. Each monomer in the complex has a substrate binding site that binds to G6P, and a catalytic coenzyme binding site that binds to NADP+/NADPH using the Rossman fold.
This is a sample scene created with SAT to by Group, and another to make of the protein.
