User:Nikhil Malvankar/Geobacter pilus structure and function

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Interactive 3D Complement in Proteopedia

Structure of novel pili evolved for extracellular translocation of microbial nanowires

Yangqi Gu, Vishok Srikanth, Ruchi Jain, Aldo I. Salazar-Morales, J. Patrick O'Brien, Sophia M. Yi, Rajesh K. Soni, Fadel A. Samatey, Sibel Ebru Yalcin, and Nikhil S. Malvankar. (journal article link here) (2020). (DOI link here)

Contents 1 Molecular Tour 2 Download 2.1 Animations for Powerpoint 3 See Also 4 Notes & References

Molecular Tour

spinqualitylabelsall models Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Previously, pili of Geobacter sulfurreducens were thought to be composed of PilA-N, a 61-amino acid protein[1][2]. Our electron cryomicroscopic structure of Geobacter sulfurreducens pili reveals them to be composed of a core of PilA-N coated with an outer surface layer of PilA-C (104 amino acids; . Here is a (front half hidden). The C-termini of PilA-N in PilA-C. The filament is assembled from . Dimer : PilA-N consists of two alpha helices, while PilA-C includes a 3-stranded beta sheet. The C-terminal protrusion of PilA-N is of PilA-C. These flaps might be open before PilA-N arrives to form a dimer, reminiscent of the flaps of HIV protease[3].

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Notes & References

1. ↑ Malvankar NS, Vargas M, Nevin K, Tremblay PL, Evans-Lutterodt K, Nykypanchuk D, Martz E, Tuominen MT, Lovley DR. Structural basis for metallic-like conductivity in microbial nanowires. MBio. 2015 Mar 3;6(2):e00084. doi: 10.1128/mBio.00084-15. PMID:25736881 doi:http://dx.doi.org/10.1128/mBio.00084-15
2. ↑ Lovley DR, Walker DJF. Geobacter Protein Nanowires. Front Microbiol. 2019 Sep 24;10:2078. doi: 10.3389/fmicb.2019.02078. eCollection , 2019. PMID:31608018 doi:http://dx.doi.org/10.3389/fmicb.2019.02078
3. ↑ Hornak V, Okur A, Rizzo RC, Simmerling C. HIV-1 protease flaps spontaneously open and reclose in molecular dynamics simulations. Proc Natl Acad Sci U S A. 2006 Jan 24;103(4):915-20. doi:, 10.1073/pnas.0508452103. Epub 2006 Jan 17. PMID:16418268 doi:http://dx.doi.org/10.1073/pnas.0508452103