Structural highlights
6ttn is a 1 chain structure with sequence from Datme. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , , , , |
| Related: | 6ttm, 6tto |
| Gene: | H6H (DATME) |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Publication Abstract from PubMed
Hyoscyamine 6beta-hydroxylase (H6H) is a bifunctional non-heme 2-oxoglutarate/Fe2+-dependent dioxygenase that catalyzes the two final steps in the biosynthesis of scopolamine. Based on high resolution crystal structures of H6H from Datura metel, detailed information on substrate binding was obtained that provided insights into the onset of the enzymatic process. In particular, the role of two prominent residues was revealed - Glu-116 that interacts with the tertiary amine located on the hyoscyamine tropane moiety and Tyr-326 that forms CH-pi hydrogen bonds with the hyoscyamine phenyl ring. The structures were used as the basis for QM/MM calculations that provided an explanation for the regioselectivity of the hydroxylation reaction on the hyoscyamine tropane moiety (C6 vs. C7) and quantified contributions of active site residues to respective barrier heights.
Regioselectivity of hyoscyamine 6beta-hydroxylase-catalysed hydroxylation as revealed by high-resolution structural information and QM/MM calculations.,Kluza A, Wojdyla Z, Mrugala B, Kurpiewska K, Porebski PJ, Niedzialkowska E, Minor W, Weiss MS, Borowski T Dalton Trans. 2020 Mar 17. doi: 10.1039/d0dt00302f. PMID:32182320[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kluza A, Wojdyla Z, Mrugala B, Kurpiewska K, Porebski PJ, Niedzialkowska E, Minor W, Weiss MS, Borowski T. Regioselectivity of hyoscyamine 6beta-hydroxylase-catalysed hydroxylation as revealed by high-resolution structural information and QM/MM calculations. Dalton Trans. 2020 Mar 17. doi: 10.1039/d0dt00302f. PMID:32182320 doi:http://dx.doi.org/10.1039/d0dt00302f
