Structural highlights
5dz6 is a 1 chain structure with sequence from Bacsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
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| Ligands: | , , , , |
| Gene: | pksC, BSU17100 (BACSU) |
| Activity: | [Acyl-carrier-protein_S-malonyltransferase [Acyl-carrier-protein] S-malonyltransferase], with EC number 2.3.1.39 |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
[PKSC_BACSU] Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the transfer of the malonyl-CoA group to the acyl-carrier-protein AcpK (Mal-AcpK).[1] [2]
Publication Abstract from PubMed
The antibiotic bacillaene is biosynthesized in Bacillus subtilis by a hybrid type 1 modular polyketide synthase/nonribosomal peptide synthetase of the trans-acyltransferase (trans-AT) class. Within this system, the essential acyl-group loading activity is provided by the action of three free-standing trans-acting acyltransferases. Here, the recombinant expression, purification and crystallization of the bacillaene synthase trans-acting acyltransferase PksC are reported. A diffraction data set has been collected from a single PksC crystal to 1.44 A resolution and the crystal was found to belong to the orthorhombic space group P2(1)2(1)2(1).
Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC.,Cuskin F, Solovyova AS, Lewis RJ, Race PR Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):464-6. doi:, 10.1107/S1744309111003484. Epub 2011 Mar 25. PMID:21505242[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Calderone CT, Kowtoniuk WE, Kelleher NL, Walsh CT, Dorrestein PC. Convergence of isoprene and polyketide biosynthetic machinery: isoprenyl-S-carrier proteins in the pksX pathway of Bacillus subtilis. Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):8977-82. Epub 2006 Jun 6. PMID:16757561 doi:http://dx.doi.org/10.1073/pnas.0603148103
- ↑ Butcher RA, Schroeder FC, Fischbach MA, Straight PD, Kolter R, Walsh CT, Clardy J. The identification of bacillaene, the product of the PksX megacomplex in Bacillus subtilis. Proc Natl Acad Sci U S A. 2007 Jan 30;104(5):1506-9. Epub 2007 Jan 18. PMID:17234808 doi:http://dx.doi.org/10.1073/pnas.0610503104
- ↑ Cuskin F, Solovyova AS, Lewis RJ, Race PR. Crystallization and preliminary X-ray analysis of the bacillaene synthase trans-acting acyltransferase PksC. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Apr 1;67(Pt 4):464-6. doi:, 10.1107/S1744309111003484. Epub 2011 Mar 25. PMID:21505242 doi:http://dx.doi.org/10.1107/S1744309111003484
