2bu3
From Proteopedia
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ACYL-ENZYME INTERMEDIATE BETWEEN ALR0975 AND GLUTATHIONE AT PH 3.4
Overview
Phytochelatin synthase (PCS) is a key enzyme for heavy-metal, detoxification in plants. PCS catalyzes the production of glutathione, (GSH)-derived peptides (called phytochelatins or PCs) that bind, heavy-metal ions before vacuolar sequestration. The enzyme can also, hydrolyze GSH and GS-conjugated xenobiotics. In the cyanobacterium Nostoc, the enzyme (NsPCS) contains only the catalytic domain of the eukaryotic, synthase and can act as a GSH hydrolase and weakly as a peptide ligase., The crystal structure of NsPCS in its native form solved at a 2.0-A, resolution shows that NsPCS is a dimer that belongs to the papain, superfamily of cysteine proteases, with a conserved catalytic machinery., Moreover, the structure of the protein solved as a complex with GSH at a, 1.4-A resolution reveals a ... [(full description)]
About this Structure
2BU3 is a [Single protein] structure of sequence from [Anabaena sp.] with CL, CA and 3GC as [ligands]. Active as [[1]], with EC number [2.3.2.15]. Full crystallographic information is available from [OCA].
Reference
A papain-like enzyme at work: native and acyl-enzyme intermediate structures in phytochelatin synthesis., Vivares D, Arnoux P, Pignol D, Proc Natl Acad Sci U S A. 2005 Dec 27;102(52):18848-53. Epub 2005 Dec 9. PMID:16339904
Page seeded by OCA on Mon Oct 29 17:50:15 2007
Categories: Anabaena sp. | Single protein | Arnoux, P. | Pignol, D. | Vivares, D. | 3GC | CA | CL | Acyl-enzyme intermediate | Alr0975 | Cysteine protease | Glutathione metabolism | Nostoc | Pcs | Phytochelatin synthase | Transferase
