| Structural highlights
Function
[MEX5_CAEEL] Functions with mex-6 to affect embryonic viability, establish soma germline asymmetry in embryos and establish plk-1, pie-1, mex-1, and pos-1 asymmetry in embryos (PubMed:10882103, PubMed:18199581, PubMed:18842813). Also affects formation of intestinal cells (PubMed:10882103). Binds to mRNA in vitro, and inhibits pgl-3-mediated P-granule formation, probably by competing with pgl-3 for binding to mRNA (PubMed:27594427).[1] [2] [3] [4]
Publication Abstract from PubMed
CCCH-type tandem zinc finger (TZF) domains are found in many RNA-binding proteins (RBPs) that regulate the essential processes of post-transcriptional gene expression and splicing through direct protein-RNA interactions. In Caenorhabditis elegans, RBPs control the translation, stability, or localization of maternal messenger RNAs required for patterning decisions before zygotic gene activation. MEX-5 (Muscle EXcess) is a C. elegans protein that leads a cascade of RBP localization events that is essential for axis polarization and germline differentiation after fertilization. Here, we report that at room temperature, the CCCH-type TZF domain of MEX-5 contains an unstructured zinc finger that folds upon binding of its RNA target. We have characterized the structure and dynamics of the TZF domain of MEX-5 and designed a variant MEX-5 in which both fingers are fully folded in the absence of RNA. Within the thermal range experienced by C. elegans, the population of the unfolded state of the TZF domain of MEX-5 varies. We observe that the TZF domain becomes less disordered at lower temperatures and more disordered at higher temperatures. However, in the temperature range in which C. elegans is fertile, when MEX-5 needs to be functional, only one of the two zinc fingers is folded.
A Disorder-to-Order Transition Mediates RNA Binding of the Caenorhabditis elegans Protein MEX-5.,Tavella D, Ertekin A, Schaal H, Ryder SP, Massi F Biophys J. 2020 Apr 21;118(8):2001-2014. doi: 10.1016/j.bpj.2020.02.032. Epub, 2020 Mar 19. PMID:32294479[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Schubert CM, Lin R, de Vries CJ, Plasterk RH, Priess JR. MEX-5 and MEX-6 function to establish soma/germline asymmetry in early C. elegans embryos. Mol Cell. 2000 Apr;5(4):671-82. PMID:10882103
- ↑ Nishi Y, Rogers E, Robertson SM, Lin R. Polo kinases regulate C. elegans embryonic polarity via binding to DYRK2-primed MEX-5 and MEX-6. Development. 2008 Feb;135(4):687-97. doi: 10.1242/dev.013425. Epub 2008 Jan 16. PMID:18199581 doi:http://dx.doi.org/10.1242/dev.013425
- ↑ Tenlen JR, Molk JN, London N, Page BD, Priess JR. MEX-5 asymmetry in one-cell C. elegans embryos requires PAR-4- and PAR-1-dependent phosphorylation. Development. 2008 Nov;135(22):3665-75. doi: 10.1242/dev.027060. Epub 2008 Oct 8. PMID:18842813 doi:http://dx.doi.org/10.1242/dev.027060
- ↑ Saha S, Weber CA, Nousch M, Adame-Arana O, Hoege C, Hein MY, Osborne-Nishimura E, Mahamid J, Jahnel M, Jawerth L, Pozniakovski A, Eckmann CR, Julicher F, Hyman AA. Polar Positioning of Phase-Separated Liquid Compartments in Cells Regulated by an mRNA Competition Mechanism. Cell. 2016 Sep 8;166(6):1572-1584.e16. doi: 10.1016/j.cell.2016.08.006. Epub 2016, Sep 1. PMID:27594427 doi:http://dx.doi.org/10.1016/j.cell.2016.08.006
- ↑ Tavella D, Ertekin A, Schaal H, Ryder SP, Massi F. A Disorder-to-Order Transition Mediates RNA Binding of the Caenorhabditis elegans Protein MEX-5. Biophys J. 2020 Apr 21;118(8):2001-2014. doi: 10.1016/j.bpj.2020.02.032. Epub, 2020 Mar 19. PMID:32294479 doi:http://dx.doi.org/10.1016/j.bpj.2020.02.032
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