2h3k
From Proteopedia
|
Solution Structure of the first NEAT domain of IsdH
Overview
During infections the pathogen Staphylococcus aureus procures the, essential nutrient iron from its host using iron-regulated surface, determinant (Isd) proteins, which scavenge heme bound iron from host, hemoproteins. Four Isd proteins are displayed in the cell wall, where they, function as receptors for host proteins and heme. Each of the receptors, contains one or more copies of a recently discovered domain called NEAT, (NEAr Transporter) that has been shown to mediate protein binding. Here we, report the three-dimensional solution structure of the NEAT domain from, the IsdH/HarA protein, which is the hemoglobin receptor in the Isd system., This is the first structure of a NEAT domain and reveals that they adopt a, beta sandwich fold that consists of two five-stranded antiparallel beta, sheets. Although unrelated at the primary sequence level, our results, indicate that NEAT domains belong to the immunoglobulin superfamily., Binding studies indicate that two IsdH/HarA NEAT domains bind a single, molecule of methemoglobin, while the distantly related NEAT domain from, the S. aureus IsdC protein binds only heme. A comparison of their primary, sequences in light of the new structure is used to predict the hemoglobin, and heme binding surfaces on NEAT domains.
About this Structure
2H3K is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Solution structure of the NEAT (NEAr Transporter) domain from IsdH/HarA: the human hemoglobin receptor in Staphylococcus aureus., Pilpa RM, Fadeev EA, Villareal VA, Wong ML, Phillips M, Clubb RT, J Mol Biol. 2006 Jul 7;360(2):435-47. Epub 2006 May 22. PMID:16762363
Page seeded by OCA on Thu Nov 8 13:31:12 2007
