Structural highlights
Disease
[PDYN_HUMAN] Spinocerebellar ataxia type 23. The disease is caused by mutations affecting the gene represented in this entry.
Function
[PDYN_HUMAN] Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress (By similarity). Dynorphin peptides differentially regulate the kappa opioid receptor. Dynorphin A(1-13) has a typical opiod activity, it is 700 times more potent than Leu-enkephalin (By similarity). Leumorphin has a typical opiod activity and may have anti-apoptotic effect.
Publication Abstract from PubMed
The structure of the dynorphin (1-13) peptide (dynorphin) bound to the human kappa opioid receptor (KOR) has been determined by liquid-state NMR spectroscopy. (1)H and (15)N chemical shift variations indicated that free and bound peptide is in fast exchange in solutions containing 1 mM dynorphin and 0.01 mM KOR. Radioligand binding indicated an intermediate-affinity interaction, with a Kd of approximately 200 nM. Transferred nuclear Overhauser enhancement spectroscopy was used to determine the structure of bound dynorphin. The N-terminal opioid signature, YGGF, was observed to be flexibly disordered, the central part of the peptide from L5 to R9 to form a helical turn, and the C-terminal segment from P10 to K13 to be flexibly disordered in this intermediate-affinity bound state. Combining molecular modeling with NMR provided an initial framework for understanding multistep activation of a G protein-coupled receptor by its cognate peptide ligand.
NMR structure and dynamics of the agonist dynorphin peptide bound to the human kappa opioid receptor.,O'Connor C, White KL, Doncescu N, Didenko T, Roth BL, Czaplicki G, Stevens RC, Wuthrich K, Milon A Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11852-7. doi:, 10.1073/pnas.1510117112. Epub 2015 Sep 8. PMID:26372966[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ O'Connor C, White KL, Doncescu N, Didenko T, Roth BL, Czaplicki G, Stevens RC, Wuthrich K, Milon A. NMR structure and dynamics of the agonist dynorphin peptide bound to the human kappa opioid receptor. Proc Natl Acad Sci U S A. 2015 Sep 22;112(38):11852-7. doi:, 10.1073/pnas.1510117112. Epub 2015 Sep 8. PMID:26372966 doi:http://dx.doi.org/10.1073/pnas.1510117112
