1uut

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spinqualitylabelsall models 1uut, resolution 2.0Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

THE NUCLEASE DOMAIN OF ADENO-ASSOCIATED VIRUS REP COMPLEXED WITH THE RBE' STEMLOOP OF THE VIRAL INVERTED TERMINAL REPEAT

Overview

Integration into a particular location in human chromosomes is a unique, property of the adeno-associated virus (AAV). This reaction requires the, viral Rep protein and AAV origin sequences. To understand how Rep, recognizes DNA, we have determined the structures of the Rep endonuclease, domain separately complexed with two DNA substrates: the Rep binding site, within the viral inverted terminal repeat and one of the terminal hairpin, arms. At the Rep binding site, five Rep monomers bind five tetranucleotide, direct repeats; each repeat is recognized by two Rep monomers from, opposing faces of the DNA. Stem-loop binding involves a protein interface, on the opposite side of the molecule from the active site where ssDNA is, cleaved. Rep therefore has three distinct binding sites within its, ... [(full description)]

About this Structure

1UUT is a [Protein complex] structure of sequences from [Adeno-associated virus - 5] with MG and CL as [ligands]. Full crystallographic information is available from [OCA].

Reference

The nuclease domain of adeno-associated virus rep coordinates replication initiation using two distinct DNA recognition interfaces., Hickman AB, Ronning DR, Perez ZN, Kotin RM, Dyda F, Mol Cell. 2004 Feb 13;13(3):403-14. PMID:14967147

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