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Publication Abstract from PubMed

Nuclear accessibility of transcription factors controls gene expression, co-regulated by Ran-dependent nuclear localization and a competitive regulatory network. Here, we reveal that nuclear import factor-facilitated transcriptional repression attenuates ribosome biogenesis under chronic salt stress. Kap114p, one of the karyopherin-betas (Kap-betas) that mediates nuclear import of yeast TATA-binding protein (yTBP), exhibits a yTBP-binding affinity four orders of magnitude greater than its counterparts and suppresses binding of yTBP with DNA. Our crystal structure of Kap114p reveals an extensively negatively charged concave surface, accounting for high-affinity basic-protein binding. KAP114 knockout in yeast leads to a high-salt growth defect, with transcriptomic analyses revealing that Kap114p modulates expression of genes associated with ribosomal biogenesis by suppressing yTBP binding to target promoters, a trans-repression mechanism we attribute to reduced nuclear Ran levels under salinity stress. Our findings reveal that Ran integrates the nuclear transport pathway and transcription regulatory network, allowing yeast to respond to environmental stresses.

Karyopherin Kap114p-mediated trans-repression controls ribosomal gene expression under saline stress.,Liao CC, Shankar S, Pi WC, Chang CC, Ahmed GR, Chen WY, Hsia KC EMBO Rep. 2020 Jun 2:e48324. doi: 10.15252/embr.201948324. PMID:32484313^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.