1lvj
From Proteopedia
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STRUCTURE OF TAR RNA COMPLEXED WITH A TAT-TAR INTERACTION NANOMOLAR INHIBITOR THAT WAS IDENTIFIED BY COMPUTATIONAL SCREENING
Overview
HIV-1 TAR RNA functions critically in viral replication by binding the, transactivating regulatory protein Tat. We recently identified several, compounds that experimentally inhibit the Tat-TAR interaction completely, at a 100 nM concentration. We used computational screening of the, 181,000-compound Available Chemicals Directory against the, three-dimensional structure of TAR [1]. Here we report the NMR-derived, structure of TAR complexed with acetylpromazine. This structure represents, a new class of compounds with good bioavailability and low toxicity that, bind with high affinity to TAR. NMR data unambiguously show that, acetylpromazine binds only to the unique 5' bulge site to which the Tat, protein binds. Specificity and affinity of binding are conferred primarily, by a network of base stacking and hydrophobic interactions., Acetylpromazine alters the structure of free TAR less than Tat peptides, and neomycin do.
About this Structure
1LVJ is a Protein complex structure of sequences from Human immunodeficiency virus 1 with PMZ as ligand. Full crystallographic information is available from OCA.
Reference
Structure of TAR RNA complexed with a Tat-TAR interaction nanomolar inhibitor that was identified by computational screening., Du Z, Lind KE, James TL, Chem Biol. 2002 Jun;9(6):707-12. PMID:12079782
Page seeded by OCA on Thu Nov 8 14:17:47 2007
