Structural highlights
Function
[XYL1_PICST] Reduces D-xylose into xylitol. Has a preference for NADPH, but can also utilize NADH as cosubstrate.[1]
Publication Abstract from PubMed
Lignocellulosic biomass, of which D-xylose accounts for approximately 35% of the total sugar, has attracted attention as a future energy source for biofuel. To elucidate molecular mechanism of D-xylose utilization, we determined the crystal structure of D-xylose reductase from Schefferzomyces stipitis (SsXR) at a 1.95 A resolution. We also determined the SsXR structure in complex with the NADPH cofactor and revealed that the protein undergoes an open/closed conformation change upon NADPH binding. The substrate binding pocket of SsXR is somewhat hydrophobic, which seems to result in low binding affinity to the substrate. Phylogenetic tree analysis showed that AKR enzymes annotated with bacterial/archaeal XRs belonged to uncharacterized AKR families and might have no XR function, and yeast/fungi derived enzymes, which belong to the same group with SsXR, can be candidates for XR to increase xylose consumption.
Structural insight into D-xylose utilization by xylose reductase from Scheffersomyces stipitis.,Son HF, Lee SM, Kim KJ Sci Rep. 2018 Nov 28;8(1):17442. doi: 10.1038/s41598-018-35703-x. PMID:30487522[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Verduyn C, Van Kleef R, Frank J, Schreuder H, Van Dijken JP, Scheffers WA. Properties of the NAD(P)H-dependent xylose reductase from the xylose-fermenting yeast Pichia stipitis. Biochem J. 1985 Mar 15;226(3):669-77. PMID:3921014
- ↑ Son HF, Lee SM, Kim KJ. Structural insight into D-xylose utilization by xylose reductase from Scheffersomyces stipitis. Sci Rep. 2018 Nov 28;8(1):17442. doi: 10.1038/s41598-018-35703-x. PMID:30487522 doi:http://dx.doi.org/10.1038/s41598-018-35703-x
