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Publication Abstract from PubMed

Successful co-crystallization of a noncovalent complex between hen egg-white lysozyme (HEWL) and the monomeric ZrIV -substituted Keggin polyoxometalate (POM) (Zr1 K1), (Et2 NH2 )3 [Zr(PW11 O39 )] (1), has been achieved, and its single-crystal X-ray structure has been determined. The dimeric ZrIV -substituted Keggin-type polyoxometalate (Zr1 K2), (Et2 NH2 )10 [Zr(PW11 O39 )2 ] (2), has been previously shown to exhibit remarkable selectivity towards HEWL hydrolysis. The reported X-ray structure shows that the hydrolytically active ZrIV -substituted Keggin POM exists as a monomeric species. Prior to hydrolysis, this monomer interacts with HEWL in the vicinity of the previously identified cleavage sites found at Trp28-Val29 and Asn44-Arg45, through water-mediated H-bonding and electrostatic interactions. Three binding sites are observed at the interface of the negatively charged Keggin POM and the positively charged regions of HEWL at: 1) Gly16, Tyr20, and Arg21; 2) Asn44, Arg45, and Asn46; and 3) Arg128.

Structural Characterization of the Complex between Hen Egg-White Lysozyme and Zr -Substituted Keggin Polyoxometalate as Artificial Protease.,Sap A, De Zitter E, Van Meervelt L, Parac-Vogt TN Chemistry. 2015 Jul 14. doi: 10.1002/chem.201501998. PMID:26179600^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.