5t6j
From Proteopedia
Structure of the MIND Complex Shows a Regulatory Focus of Yeast Kinetochore Assembly
Structural highlights
Function[SPC24_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.[1] [2] [3] [DSN1_YEAST] Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules.[4] [5] [SPC25_YEAST] Acts as a component of the essential kinetochore-associated NDC80 complex, which is involved in chromosome segregation and spindle checkpoint activity.[6] [7] Publication Abstract from PubMedKinetochores connect centromeric nucleosomes with mitotic-spindle microtubules through conserved, cross-interacting protein subassemblies. In budding yeast, the heterotetrameric MIND complex (Mtw1, Nnf1, Nsl1, Dsn1), ortholog of the metazoan Mis12 complex, joins the centromere-proximal components, Mif2 and COMA, with the principal microtubule-binding component, the Ndc80 complex (Ndc80C). We report the crystal structure of Kluyveromyces lactis MIND and examine its partner interactions, to understand the connection from a centromeric nucleosome to a much larger microtubule. MIND resembles an elongated, asymmetric Y; two globular heads project from a coiled-coil shaft. An N-terminal extension of Dsn1 from one head regulates interactions of the other head, blocking binding of Mif2 and COMA. Dsn1 phosphorylation by Ipl1/Aurora B relieves this autoinhibition, enabling MIND to join an assembling kinetochore. A C-terminal extension of Dsn1 recruits Ndc80C to the opposite end of the shaft. The structure and properties of MIND show how it integrates phospho-regulatory inputs for kinetochore assembly and disassembly. Structure of the MIND Complex Defines a Regulatory Focus for Yeast Kinetochore Assembly.,Dimitrova YN, Jenni S, Valverde R, Khin Y, Harrison SC Cell. 2016 Nov 3;167(4):1014-1027.e12. doi: 10.1016/j.cell.2016.10.011. Epub 2016, Oct 27. PMID:27881300[8] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Baker's yeast | Large Structures | Dimitrova, Y | Harrison, S C | Jenni, S | Khin, Y | Valverde, R | Cell cycle | Chromosome | Complex | Kinetochore | Mind | Mis12 | Mtw1 | Ndc80 | Nuf2 | Segregation | Spc24 | Spc25
