Structural highlights
Function
[ATC6_YEAST] Mediates manganese transport into the endoplasmic reticulum. The ATPase activity is required for cellular manganese homeostasis.[1]
Publication Abstract from PubMed
Organelle identity depends on protein composition. How mistargeted proteins are selectively recognized and removed from organelles is incompletely understood. Here, we found that the orphan P5A-adenosine triphosphatase (ATPase) transporter ATP13A1 (Spf1 in yeast) directly interacted with the transmembrane segment (TM) of mitochondrial tail-anchored proteins. P5A-ATPase activity mediated the extraction of mistargeted proteins from the endoplasmic reticulum (ER). Cryo-electron microscopy structures of Saccharomyces cerevisiae Spf1 revealed a large, membrane-accessible substrate-binding pocket that alternately faced the ER lumen and cytosol and an endogenous substrate resembling an alpha-helical TM. Our results indicate that the P5A-ATPase could dislocate misinserted hydrophobic helices flanked by short basic segments from the ER. TM dislocation by the P5A-ATPase establishes an additional class of P-type ATPase substrates and may correct mistakes in protein targeting or topogenesis.
The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase.,McKenna MJ, Sim SI, Ordureau A, Wei L, Harper JW, Shao S, Park E Science. 2020 Sep 25;369(6511). pii: 369/6511/eabc5809. doi:, 10.1126/science.abc5809. PMID:32973005[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Cohen Y, Megyeri M, Chen OC, Condomitti G, Riezman I, Loizides-Mangold U, Abdul-Sada A, Rimon N, Riezman H, Platt FM, Futerman AH, Schuldiner M. The yeast p5 type ATPase, spf1, regulates manganese transport into the endoplasmic reticulum. PLoS One. 2013 Dec 31;8(12):e85519. doi: 10.1371/journal.pone.0085519., eCollection 2013. PMID:24392018 doi:http://dx.doi.org/10.1371/journal.pone.0085519
- ↑ McKenna MJ, Sim SI, Ordureau A, Wei L, Harper JW, Shao S, Park E. The endoplasmic reticulum P5A-ATPase is a transmembrane helix dislocase. Science. 2020 Sep 25;369(6511). pii: 369/6511/eabc5809. doi:, 10.1126/science.abc5809. PMID:32973005 doi:http://dx.doi.org/10.1126/science.abc5809
