Structural highlights
Function
[PRK6_ARATH] Key receptor for sensing species-specific attractants in cooperation with other pollen receptor-like kinases. Essential for pollen tube reorientation toward attractant peptides.[1] [LUR12_ARATH] Pollen tube attractants guiding pollen tubes to the ovular micropyle. Attracts specifically pollen tubes from A.thaliana, but not those from A.lyrata (PubMed:23271953). Triggers endocytosis of MDIS1 in the pollen tube tip (PubMed:26863186).[2] [3]
Publication Abstract from PubMed
Transportation of the immobile sperms directed by pollen tubes to the ovule-enclosed female gametophytes is important for plant sexual reproduction. The defensin-like (DEFL) cysteine-rich peptides (CRPs) LUREs play an essential role in pollen tube attraction to the ovule, though their receptors still remain controversial. Here we provide several lines of biochemical evidence showing that the extracellular domain of the leucine-rich repeat receptor kinase (LRR-RK) PRK6 from Arabidopsis thaliana directly interacts with AtLURE1 peptides. Structural study reveals that a C-terminal loop of the LRR domain (AtPRK6(LRR)) is responsible for recognition of AtLURE1.2, mediated by a set of residues largely conserved among PRK6 homologs from Arabidopsis lyrata and Capsella rubella, supported by in vitro mutagenesis and semi-in-vivo pollen tube growth assays. Our study provides evidence showing that PRK6 functions as a receptor of the LURE peptides in A. thaliana and reveals a unique ligand recognition mechanism of LRR-RKs.
Structural basis for receptor recognition of pollen tube attraction peptides.,Zhang X, Liu W, Nagae TT, Takeuchi H, Zhang H, Han Z, Higashiyama T, Chai J Nat Commun. 2017 Nov 6;8(1):1331. doi: 10.1038/s41467-017-01323-8. PMID:29109411[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Takeuchi H, Higashiyama T. Tip-localized receptors control pollen tube growth and LURE sensing in Arabidopsis. Nature. 2016 Mar 10;531(7593):245-8. doi: 10.1038/nature17413. PMID:26961657 doi:http://dx.doi.org/10.1038/nature17413
- ↑ Takeuchi H, Higashiyama T. A species-specific cluster of defensin-like genes encodes diffusible pollen tube attractants in Arabidopsis. PLoS Biol. 2012;10(12):e1001449. doi: 10.1371/journal.pbio.1001449. Epub 2012 Dec, 18. PMID:23271953 doi:http://dx.doi.org/10.1371/journal.pbio.1001449
- ↑ Wang T, Liang L, Xue Y, Jia PF, Chen W, Zhang MX, Wang YC, Li HJ, Yang WC. A receptor heteromer mediates the male perception of female attractants in plants. Nature. 2016 Mar 10;531(7593):241-4. doi: 10.1038/nature16975. Epub 2016 Feb 10. PMID:26863186 doi:http://dx.doi.org/10.1038/nature16975
- ↑ Zhang X, Liu W, Nagae TT, Takeuchi H, Zhang H, Han Z, Higashiyama T, Chai J. Structural basis for receptor recognition of pollen tube attraction peptides. Nat Commun. 2017 Nov 6;8(1):1331. doi: 10.1038/s41467-017-01323-8. PMID:29109411 doi:http://dx.doi.org/10.1038/s41467-017-01323-8
