2f4x
From Proteopedia
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NMR Solution of HIV-1 Lai Kissing Complex
Overview
Dimerization of genomic RNA is directly related with the event of, encapsidation and maturation of the virion. The initiating sequence of the, dimerization is a short autocomplementary region in the hairpin loop SL1., We describe here a new solution structure of the RNA dimerization, initiation site (DIS) of HIV-1(Lai). NMR pulsed field-gradient spin-echo, techniques and multidimensional heteronuclear NMR spectroscopy indicate, that this structure is formed by two hairpins linked by six Watson-Crick, GC base pairs. Hinges between the stems and the loops are stabilized by, intra and intermolecular interactions involving the A8, A9 and A16, adenines. The coaxial alignment of the three A-type helices present in the, structure is supported by previous crystallography analysis but the A8 and, A9 adenines are found in a bulged in position. These data suggest the, existence of an equilibrium between bulged in and bulged out conformations, in solution.
About this Structure
2F4X is a Protein complex structure of sequences from [1]. Full crystallographic information is available from OCA.
Reference
A new NMR solution structure of the SL1 HIV-1Lai loop-loop dimer., Kieken F, Paquet F, Brule F, Paoletti J, Lancelot G, Nucleic Acids Res. 2006 Jan 12;34(1):343-52. Print 2006. PMID:16410614
Page seeded by OCA on Thu Nov 8 14:46:21 2007
Categories: Protein complex | Brule, F. | Kieken, F. | Lancelot, G. | Paoletti, J. | Paquet, F. | Hairpin | Kissing complex | Rna | Sl1
