2nuo
From Proteopedia
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Crystal structure of a complex of griffithsin with glucose
Overview
Crystal structures of complexes of an antiviral lectin griffithsin (GRFT), with glucose and N-acetylglucosamine were solved and refined at high, resolution. In both complexes, all six monosaccharide-binding sites of, GRFT were occupied and the mode of binding was similar to that of mannose., In our previous attempts to obtain a complex with N-acetylglucosamine by, soaking, only a single site was occupied; thus, cocrystallization was, clearly superior despite lower concentration of the ligand. Isothermal, titration calorimetric experiments with N-acetylglucosamine, glucose, and, mannose provided enthalpic evidence of distinct binding differences, between the three monosaccharides. A comparison of the mode of binding of, different monosaccharides is discussed in the context of the antiviral, activity of GRFT, based on specific binding to high-mannose-containing, complex carbohydrates found on viral envelopes.
About this Structure
2NUO is a Single protein structure of sequence from Griffithsia with GLC, SO4 and EDO as ligands. Full crystallographic information is available from OCA.
Reference
Crystallographic studies of the complexes of antiviral protein griffithsin with glucose and N-acetylglucosamine., Ziolkowska NE, Shenoy SR, O'Keefe BR, Wlodawer A, Protein Sci. 2007 Jul;16(7):1485-9. Epub 2007 Jun 13. PMID:17567736
Page seeded by OCA on Thu Nov 8 14:53:29 2007
Categories: Griffithsia | Single protein | Wlodawer, A. | Ziolkowska, N.E. | EDO | GLC | SO4 | Domain swapping | Griffithsin | Hiv | Lectins | Mannose binding | Sars | Sugar binding protein
