484d
From Proteopedia
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SOLUTION STRUCTURE OF HIV-1 REV PEPTIDE-RNA APTAMER COMPLEX
Overview
BACKGROUND: The biological function of several viral and bacteriophage, proteins, and their arginine-rich subdomains, involves RNA-mediated, interactions. It has been shown recently that bound peptides adopt either, beta-hairpin or alpha-helical conformations in viral and phage peptide-RNA, complexes. We have compared the structures of the arginine-rich peptide, domain of HIV-1 Rev bound to two RNA aptamers to determine whether RNA, architecture can dictate the conformations of a bound peptide. RESULTS:, The core-binding segment of the HIV-1 Rev peptide class II RNA aptamer, complex spans the two-base bulge and hairpin loop of the bound RNA and the, carboxy-terminal segment of the bound peptide. The bound peptide is, anchored in place by backbone and sidechain intermolecular hydrogen, bonding and van der Waals stacking interactions. One of the bulge bases, participates in U*(A*U) base triple formation, whereas the other is looped, out and flaps over the bound peptide in the complex. The seven-residue, hairpin loop is closed by a sheared G*A mismatch pair with several, pyrimidines looped out of the hairpin fold. CONCLUSIONS: Our structural, studies establish that RNA architecture dictates whether the same HIV-1, Rev peptide folds into an extended or alpha-helical conformation on, complex formation. Arginine-rich peptides can therefore adapt distinct, secondary folds to complement the tertiary folds of their RNA targets., This contrasts with protein-RNA complexes in which elements of RNA, secondary structure adapt to fit within the tertiary folds of their, protein targets.
About this Structure
484D is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
RNA architecture dictates the conformations of a bound peptide., Ye X, Gorin A, Frederick R, Hu W, Majumdar A, Xu W, McLendon G, Ellington A, Patel DJ, Chem Biol. 1999 Sep;6(9):657-69. PMID:10467126
Page seeded by OCA on Thu Nov 8 14:58:48 2007
