Structural highlights
Function
[CTBP1_CAEEL] Binds DNA and represses gene expression. Plays a role in regulation of life span, possibly by regulating transcription of genes important for lipid metabolism.[1] [2]
Publication Abstract from PubMed
The THAP (Thanatos-associated protein) domain is a recently discovered zinc-binding domain found in proteins involved in transcriptional regulation, cell-cycle control, apoptosis and chromatin modification. It contains a single zinc atom ligated by cysteine and histidine residues within a Cys-X(2-4)-Cys-X(35-53)-Cys-X(2)-His consensus. We have determined the NMR solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP) and show that it adopts a fold containing a treble clef motif, bearing similarity to the zinc finger-associated domain (ZAD) from Drosophila Grauzone. The CtBP THAP domain contains a large, positively charged surface patch and we demonstrate that this domain can bind to double-stranded DNA in an electrophoretic mobility-shift assay. These data, together with existing reports, indicate that THAP domains might exhibit a functional diversity similar to that observed for classical and GATA-type zinc fingers.
Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP).,Liew CK, Crossley M, Mackay JP, Nicholas HR J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Nicholas HR, Lowry JA, Wu T, Crossley M. The Caenorhabditis elegans protein CTBP-1 defines a new group of THAP domain-containing CtBP corepressors. J Mol Biol. 2008 Jan 4;375(1):1-11. Epub 2007 Oct 22. PMID:18005989 doi:http://dx.doi.org/10.1016/j.jmb.2007.10.041
- ↑ Chen S, Whetstine JR, Ghosh S, Hanover JA, Gali RR, Grosu P, Shi Y. The conserved NAD(H)-dependent corepressor CTBP-1 regulates Caenorhabditis elegans life span. Proc Natl Acad Sci U S A. 2009 Feb 3;106(5):1496-501. doi:, 10.1073/pnas.0802674106. Epub 2009 Jan 21. PMID:19164523 doi:http://dx.doi.org/10.1073/pnas.0802674106
- ↑ Liew CK, Crossley M, Mackay JP, Nicholas HR. Solution structure of the THAP domain from Caenorhabditis elegans C-terminal binding protein (CtBP). J Mol Biol. 2007 Feb 16;366(2):382-90. Epub 2006 Nov 18. PMID:17174978 doi:S0022-2836(06)01598-1
