Structural highlights
Function
[SKN1_CAEEL] Required to specify the fate of ventral blastomeres in the early embryo, and postembryonically for the development of the intestine. Directly regulates expression of zygotically expressed med-1 and med-2 to direct mesendoderm development.[1] [2]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The DNA-binding domain of Skn-1, a developmental transcription factor that specifies mesoderm in C. elegans, is shown by X-ray crystallography to have a novel fold in which a compact, monomeric, four-helix unit organizes two DNA-contact elements. At the C-terminus, a helix extends from the domain to occupy the major groove of DNA in a manner similar to bZip proteins. Skn-1, however, lacks the leucine zipper found in all bZips. Additional contacts with the DNA are made by a short basic segment at the N-terminus of the domain, reminiscent of the 'homeodomain arm'.
A new DNA-binding motif in the Skn-1 binding domain-DNA complex.,Rupert PB, Daughdrill GW, Bowerman B, Matthews BW Nat Struct Biol. 1998 Jun;5(6):484-91. PMID:9628487[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bowerman B, Eaton BA, Priess JR. skn-1, a maternally expressed gene required to specify the fate of ventral blastomeres in the early C. elegans embryo. Cell. 1992 Mar 20;68(6):1061-75. PMID:1547503
- ↑ Maduro MF, Meneghini MD, Bowerman B, Broitman-Maduro G, Rothman JH. Restriction of mesendoderm to a single blastomere by the combined action of SKN-1 and a GSK-3beta homolog is mediated by MED-1 and -2 in C. elegans. Mol Cell. 2001 Mar;7(3):475-85. PMID:11463373
- ↑ Rupert PB, Daughdrill GW, Bowerman B, Matthews BW. A new DNA-binding motif in the Skn-1 binding domain-DNA complex. Nat Struct Biol. 1998 Jun;5(6):484-91. PMID:9628487
