Structural highlights
Function
[WRKY4_ARATH] Transcription factor. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element (By similarity).
Publication Abstract from PubMed
The WRKY family transcription factors regulate plant-specific reactions that are mostly related to biotic and abiotic stresses. They share the WRKY domain that recognizes a DNA element (TTCAC[C/T]), termed W-box, in target genes. Here we determined the solution structure of the C-terminal WRKY domain of Arabidopsis WRKY4 in complex with the W-box DNA by NMR. A four-stranded beta-sheet enters the major groove of DNA in an atypical mode termed the beta-wedge, where the sheet is nearly perpendicular to the DNA helical axis. Residues in the conserved WRKYGQK motif contact with DNA bases mainly through extensive apolar contacts with thymine methyl groups. The importance of these contacts was verified by substituting the relevant T bases with U and by surface plasmon resonance (SPR) analyses of DNA binding.
Structural basis for sequence-specific DNA recognition by an Arabidopsis WRKY transcription factor.,Yamasaki K, Kigawa T, Watanabe S, Inoue M, Yamasaki T, Seki M, Shinozaki K, Yokoyama S J Biol Chem. 2012 Jan 4. PMID:22219184[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Yamasaki K, Kigawa T, Watanabe S, Inoue M, Yamasaki T, Seki M, Shinozaki K, Yokoyama S. Structural basis for sequence-specific DNA recognition by an Arabidopsis WRKY transcription factor. J Biol Chem. 2012 Jan 4. PMID:22219184 doi:10.1074/jbc.M111.279844
