1auk

From Proteopedia

HUMAN ARYLSULFATASE A

Overview

Human lysosomal arylsulfatase A (ASA) is a prototype member of the, sulfatase family. These enzymes require the posttranslational oxidation of, the -CH2SH group of a conserved cysteine to an aldehyde, yielding a, formylglycine. Without this modification sulfatases are catalytically, inactive, as revealed by a lysosomal storage disorder known as multiple, sulfatase deficiency. The 2.1 A resolution X-ray crystal structure shows, an ASA homooctamer composed of a tetramer of dimers, (alpha 2)4. The, alpha/beta fold of the monomer has significant structural analogy to, another hydrolytic enzyme, the alkaline phosphatase, and superposition of, these two structures shows that the active centers are located in largely, identical positions. The functionally essential formylglycine is located, in a positively charged pocket and acts as ligand to an octahedrally, coordinated metal ion interpreted as Mg2+. The electron density at the, formylglycine suggests the presence of a 2-fold disordered aldehyde group, with the possible contribution of an aldehyde hydrate, -CH(OH)2, with, gem-hydroxyl groups. In the proposed catalytic mechanism, the aldehyde, accepts a water molecule to form a hydrate. One of the two hydroxyl groups, hydrolyzes the substrate sulfate ester via a transesterification step, resulting in a covalent intermediate. The second hydroxyl serves to, eliminate sulfate under inversion of configuration through C-O cleavage, and reformation of the aldehyde. This study provides the structural basis, for understanding a novel mechanism of ester hydrolysis and explains the, functional importance of the unusually modified amino acid.

Disease

Known disease associated with this structure: Metachromatic leukodystrophy OMIM:[607574]

About this Structure

1AUK is a Single protein structure of sequence from Homo sapiens with MG as ligand. Active as Cerebroside-sulfatase, with EC number 3.1.6.8 Structure known Active Site: ACT. Full crystallographic information is available from OCA.

Reference

Crystal structure of human arylsulfatase A: the aldehyde function and the metal ion at the active site suggest a novel mechanism for sulfate ester hydrolysis., Lukatela G, Krauss N, Theis K, Selmer T, Gieselmann V, von Figura K, Saenger W, Biochemistry. 1998 Mar 17;37(11):3654-64. PMID:9521684

Page seeded by OCA on Mon Nov 12 16:02:17 2007