1awz

From Proteopedia

3D SOLUTION STRUCTURE OF HUMAN ANGIOGENIN DETERMINED BY 1H, 15N NMR SPECTROSCOPY, 30 STRUCTURES

Overview

Human angiogenin is a member of the pancreatic ribonuclease superfamily, that induces blood vessel formation. Its three-dimensional solution, structure has been determined to high resolution by heteronuclear NMR, spectroscopy. 30 structures were calculated, based on a total of 1441, assigned NOE correlations, 64 coupling constants and 50 hydrogen bonds., The backbone atomic rms difference from the mean coordinates is 0.067 +/-, 0.012 nm and 0.13 nm from the previously determined crystal structure. The, side-chain of Gln117 was found to obstruct the active site as observed in, the crystal state. There was no evidence of an alternative open form of, angiogenin, although two sets of chemical shifts were observed for some, residues, mainly around the active site and in the C-terminal segment. The, topology of the ribonucleolytic active site is described with a particular, emphasis on the conformation and protonation of active-site His residues., The side-chain of His114 adopts two main conformations in solution. In, contrast to pancreatic ribonuclease A, His13 was shown to be more basic, than His114, with pKa values of 6.65 and 6.05 respectively. The His47, residue is located in an environment very resistant to protonation with a, pKa lower than 4.

Disease

Known diseases associated with this structure: Amyotrophic lateral sclerosis, susceptibility to OMIM:[105850]

About this Structure

1AWZ is a Single protein structure of sequence from Homo sapiens. Structure known Active Sites: NLS, RBS and RIB. Full crystallographic information is available from OCA.

Reference

Three-dimensional solution structure of human angiogenin determined by 1H,15N-NMR spectroscopy--characterization of histidine protonation states and pKa values., Lequin O, Thuring H, Robin M, Lallemand JY, Eur J Biochem. 1997 Dec 15;250(3):712-26. PMID:9461294

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