2iuc
From Proteopedia
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STRUCTURE OF ALKALINE PHOSPHATASE FROM THE ANTARCTIC BACTERIUM TAB5
Overview
Alkaline phosphatases (APs) are non-specific phosphohydrolases that are, widely used in molecular biology and diagnostics. We describe the, structure of the cold active alkaline phosphatase from the Antarctic, bacterium TAB5 (TAP). The fold and the active site geometry are conserved, with the other AP structures, where the monomer has a large central, beta-sheet enclosed by alpha-helices. The dimer interface of TAP is, relatively small, and only a single loop from each monomer replaces the, typical crown domain. The structure also has typical cold-adapted, features; lack of disulfide bridges, low number of salt-bridges, and a, loose dimer interface that completely lacks charged interactions. The, dimer interface is more hydrophobic than that of the Escherichia coli AP, and the ... [(full description)]
About this Structure
2IUC is a [Protein complex] structure of sequences from [Antarctic bacterium tab5] with ZN, PO4, MG and CAC as [ligands]. Active as [[1]], with EC number [3.1.3.1]. Full crystallographic information is available from [OCA].
Reference
Crystal structure of alkaline phosphatase from the Antarctic bacterium TAB5., Wang E, Koutsioulis D, Leiros HK, Andersen OA, Bouriotis V, Hough E, Heikinheimo P, J Mol Biol. 2007 Mar 2;366(4):1318-31. Epub 2006 Dec 2. PMID:17198711
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