This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1b3a
From Proteopedia
|
TOTAL CHEMICAL SYNTHESIS AND HIGH-RESOLUTION CRYSTAL STRUCTURE OF THE POTENT ANTI-HIV PROTEIN AOP-RANTES
Contents |
Overview
BACKGROUND: RANTES is a CC-type chemokine protein that acts as a, chemoattractant for several kinds of leukocytes, playing an important, pro-inflammatory role. Entry of human immunodeficiency virus-1 (HIV-1), into cells depends on the chemokine receptor CCR5. RANTES binds CCR5 and, inhibits HIV-1 entry into peripheral blood cells. Interaction with, chemokine receptors involves a distinct set of residues at the amino, terminus of RANTES. This finding was utilized in the development of a, chemically modified aminooxypentane derivative of RANTES, AOP-RANTES, that, was originally produced from the recombinant protein using semisynthetic, methods. RESULTS: AOP-RANTES has been produced by a novel total chemical, synthesis that provides efficient, direct access to large amounts of this, anti-HIV protein analog. The crystal structure of chemically synthesized, AOP-RANTES has been solved and refined at 1.6 A resolution. The protein is, a dimer, with the amino-terminal pentane oxime moiety clearly defined., CONCLUSIONS: Total chemical synthesis of AOP-RANTES provides a convenient, method of producing the multi-milligram quantities of this protein needed, to investigate the molecular basis of receptor binding and antiviral, activity. This work provides the first truly high-resolution structure of, a RANTES protein, although the structure of RANTES was known from previous, nuclear magnetic resonance (NMR) determinations.
Disease
Known diseases associated with this structure: HIV-1 disease, delayed progression of OMIM:[187011], HIV-1 disease, rapid progression of OMIM:[187011]
About this Structure
1B3A is a Single protein structure of sequence from [1] with SO4 and AOP as ligands. Full crystallographic information is available from OCA.
Reference
Total chemical synthesis and high-resolution crystal structure of the potent anti-HIV protein AOP-RANTES., Wilken J, Hoover D, Thompson DA, Barlow PN, McSparron H, Picard L, Wlodawer A, Lubkowski J, Kent SB, Chem Biol. 1999 Jan;6(1):43-51. PMID:9889151
Page seeded by OCA on Mon Nov 12 16:05:09 2007
Categories: Single protein | Barlow, P.N. | Hoover, D. | Kent, S.B.H. | Lubkowski, J. | Mcsparron, H. | Picard, L. | Thompson, D.A. | Wilken, J. | Wlodawer, A. | AOP | SO4 | Chemical protein synthesis | Chemokine | Crystal structure | Hiv-1 | Rantes
