Structure of a C1/C4-oxidizing AA9 lytic polysaccharide monooxygenase from the thermophilic fungus Malbranchea cinnamomea
Scott Mazurkewich, Andrea Seveso, Silvia Hüttner, Gisela Brändén, Johan Larsbrink [1]
Molecular Tour
The thermophilic fungus Malbranchea cinnamomea encodes eight lytic polysaccharide monooxygenases (LPMOs) from the Auxiliary Activities family 9 (AA9), four of which have been shown to oxidatively cleave various polysaccharides. Here we solved the structure of McAA9F, which is a C1/C4-oxidizing LPMO able to cleave both crystalline and soluble glycans. The structure reveals that McAA9F has an overall similar fold as other solved AA9 enzymes, but also has different loop structures than what have previously been linked to activity on soluble oligosaccharides. The structure further contains a rare succinimide substitution that has not been seen in other LPMOs.
References
- ↑ Mazurkewich S, Seveso A, Huttner S, Branden G, Larsbrink J. Structure of a C1/C4-oxidizing AA9 lytic polysaccharide monooxygenase from the thermophilic fungus Malbranchea cinnamomea. Acta Crystallogr D Struct Biol. 2021 Aug 1;77(Pt 8):1019-1026. doi:, 10.1107/S2059798321006628. Epub 2021 Jul 29. PMID:34342275 doi:http://dx.doi.org/10.1107/S2059798321006628
