1bzy

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spinqualitylabelsall models 1bzy, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

HUMAN HGPRTASE WITH TRANSITION STATE INHIBITOR

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The structure of human HGPRT bound to the transition-state analog, immucillinGP and Mg2+-pyrophosphate has been determined to 2.0 A, resolution. ImmucillinGP was designed as a stable analog with the, stereoelectronic features of the transition state. Bound inhibitor at the, catalytic site indicates that the oxocarbenium ion of the transition state, is stabilized by neighboring-group participation from MgPPi and O5'. A, short hydrogen bond forms between Asp 137 and the purine ring analog. Two, Mg2+ ions sandwich the pyrophosphate and contact both hydroxyls of the, ribosyl analog. The transition-state analog is shielded from bulk solvent, by a catalytic loop that moves approximately 25 A to cover the active site, and becomes an ordered antiparallel beta-sheet.

Disease

Known diseases associated with this structure: HPRT-related gout OMIM:[308000], Lesch-Nyhan syndrome, 300322, OMIM:[308000]

About this Structure

1BZY is a Single protein structure of sequence from Homo sapiens with MG, IMU and POP as ligands. Active as Hypoxanthine phosphoribosyltransferase, with EC number 2.4.2.8 Full crystallographic information is available from OCA.

Reference

The 2.0 A structure of human hypoxanthine-guanine phosphoribosyltransferase in complex with a transition-state analog inhibitor., Shi W, Li CM, Tyler PC, Furneaux RH, Grubmeyer C, Schramm VL, Almo SC, Nat Struct Biol. 1999 Jun;6(6):588-93. PMID:10360366

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