Sandbox Reserved 1697

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Function of your protein

The function of is dephosphorylating inositol, used in inositol signaling, the substrate of the enzyme is IP3 and the product is IP2. The amino acids of the protein hold onto the magnesium metal ions that then hold onto the water molecule, this allows for it to take off a phosphate.

Biological relevance and broader implications

The protein 7KIR is a protype member of a family of enzymes involved in dephosphorylation IP3. The two metal ions, Magnesium and Lithium, are particularly important in activation and inhibition of inositol signaling. Since these enzymes are seen in many different organisms, with slight mutations in the motif of the enzyme without change in function, understanding the mechanics of enzyme inhibition in this metabolic pathway would allow a better understanding of metabolic pathways.

Important amino acids

In this protein the amino acids do not interact with the substrate itself, instead they interact with the metal ions. When magnesium is present, and the protein therefore activated, the amino acids will hold onto the magnesium ions. These magnesium ions that interact with the inositol and water so that it can dephosphorylate the substrate. The motif of enzymes in this family is DPIDXT. In this particular protein the motif is . There are some mutations in the enzyme but the overall function of the protein is still the same.

Structural highlights

This protein is a mutation of 1INP, it has thirteen structural elements. The amino acids in the bind to the substrate all participate in hydrogen bonding to the substrate and ligand. The structure is , the betta sheets allow for twisting of the molecule so that the ligand better fits within the enzyme. The helices allow for hydrogen bonding throughout to stabilize the structure with assistance from metal ions; specifically magnesium ions. ^[3]

Other important features

The ligand of the protein is known as , with a PDB ID of 2IP. The cyclic structure of the ligand could possibly assist with binding the substrate, the hydrogen bonding with various amino acids allows for many interactions with the enzyme.

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