1drf
From Proteopedia
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CRYSTAL STRUCTURE OF HUMAN DIHYDROFOLATE REDUCTASE COMPLEXED WITH FOLATE
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Overview
The crystal structure of recombinant human dihydrofolate reductase with, folate bound in the active site has been determined and the structural, model refined at 0.2-nm resolution. Preliminary studies of the binding of, the inhibitors methotrexate and trimethoprim to the human apoenzyme have, been performed at 0.35-nm resolution. The conformations of the chemically, very similar ligands folate and methotrexate, one a substrate the other a, potent inhibitor, differ substantially in that their pteridine rings are, in inverse orientations relative to their p-aminobenzoyl-L-glutamate, moieties. Methotrexate binding is similar to that previously observed in, two bacterial enzymes but is quite different from that observed in the, enzyme from a mouse lymphoma cell line [Stammers et al. (1987) FEBS Lett., 218, 178-184]. The geometry of the polypeptide chain around the folate, binding site in the human enzyme is not consistent with conclusions, previously drawn with regard to the species selectivity of the inhibitor, trimethoprim [Matthews et al. (1985) J. Biol. Chem. 260, 392-399].
Disease
Known disease associated with this structure: Anemia, megaloblastic, due to DHFR deficiency (1) OMIM:[126060]
About this Structure
1DRF is a Single protein structure of sequence from Homo sapiens with SO4 and FOL as ligands. Active as Dihydrofolate reductase, with EC number 1.5.1.3 Full crystallographic information is available from OCA.
Reference
Crystal structure of human dihydrofolate reductase complexed with folate., Oefner C, D'Arcy A, Winkler FK, Eur J Biochem. 1988 Jun 1;174(2):377-85. PMID:3383852
Page seeded by OCA on Mon Nov 12 16:35:10 2007
