Serine palmitoyltransferase

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models Dimeric prokaryotic serine palmitoyltransferase complex with serine and cofactor pyridoxal phosphate 3a2b Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image Function Serine palmitoyltransferase (SPT) catalyzes the conversion of palmitoyl-CoA and serine to CoA and dehydro-sphinganine. This reaction is part of sphingosine biosynthesis. Pyridoxal phosphate (PLP) is a cofactor in the reaction. The prokaryotic SPT is a soluble homodimer while the eukaryotic one is heterodimeric and is anchored in the endoplasmic reticulum[1]. Structural highlights The of SPT contains a [2]. Water molecules are shown as red spheres.

3D structures of serine palmitoyltransferase

Serine palmitoyltransferase 3D structures Updated on 15-March-2022

7k0l – hSPT1 + SPT2 + SPT small subunit A + antibiotic + PLP – human – Cryo EM
7k0k – hSPT1 + SPT2 + SPT small subunit A + sphinganine derivative – Cryo EM
7k0i, 7k0j – hSPT1 + SPT2 + SPT small subunit A – Cryo EM
7k0q – hSPT1 + SPT2 + SPT small subunit A + ORM1-like protein + antibiotic + PLP – Cryo EM
6m4n, 6m4o, 7k0m, 7k0n, 7k0o, [7k0p]] – hSPT1 + SPT2 + SPT small subunit A + ORM1-like protein + PLP – Cryo EM
7cqi, 7cqk – hSPT1 + SPT2 + SPT small subunit A + ORM1-like protein + PLP derivative + substrate – Cryo EM
2jg2 – PpSPT + aldimine – Pseudomonas paucimobilis
2jgt – PpSPT
2w8j - SpSPT + aldimine – Sphingomonas paucimobilis
2w8t, 2w8u, 2w8v, 2w8w - SpSPT (mutant) + aldimine
4bmk - SpSPT (mutant) + antibiotic + PLP
2xbn – SpSPT + pyridoxamine phosphate
3a2b – SPT + pyridoxal phosphate + serine – Sphingobacterium multivorum
2x8u - SwSPT + pyridoxal derivative – Sphingomonas wittichii
7e13 - SPT + PLP – Erigeron canadensis

References

1. ↑ Hanada K. Serine palmitoyltransferase, a key enzyme of sphingolipid metabolism. Biochim Biophys Acta. 2003 Jun 10;1632(1-3):16-30. PMID:12782147
2. ↑ Ikushiro H, Islam MM, Okamoto A, Hoseki J, Murakawa T, Fujii S, Miyahara I, Hayashi H. Structural insights into the enzymatic mechanism of serine palmitoyltransferase from Sphingobacterium multivorum. J Biochem. 2009 Oct;146(4):549-62. Epub 2009 Jun 29. PMID:19564159 doi:10.1093/jb/mvp100