2rrk

Structural highlights

Function

[NUDG_ECOLI] Hydrolase with a preference for pyrimidine substrates. Has high activity with 5-methyl-dCTP, and much lower activity with CTP, dCTP, 5-hydroxy-dCTP, 2-hydroxy-dATP and 8-hydroxy-dGTP.^{[1] [2] [3]}

References

1. ↑ O'Handley SF, Dunn CA, Bessman MJ. Orf135 from Escherichia coli Is a Nudix hydrolase specific for CTP, dCTP, and 5-methyl-dCTP. J Biol Chem. 2001 Feb 23;276(8):5421-6. Epub 2000 Oct 26. PMID:11053429 doi:http://dx.doi.org/10.1074/jbc.M004100200
2. ↑ Fujikawa K, Kasai H. The oxidized pyrimidine ribonucleotide, 5-hydroxy-CTP, is hydrolyzed efficiently by the Escherichia coli recombinant Orf135 protein. DNA Repair (Amst). 2002 Jul 17;1(7):571-6. PMID:12509230
3. ↑ Iida E, Satou K, Mishima M, Kojima C, Harashima H, Kamiya H. Amino acid residues involved in substrate recognition of the Escherichia coli Orf135 protein. Biochemistry. 2005 Apr 19;44(15):5683-9. PMID:15823026 doi:http://dx.doi.org/10.1021/bi048071o