1eaj
From Proteopedia
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DIMERIC STRUCTURE OF THE COXSACKIE VIRUS AND ADENOVIRUS RECEPTOR D1 DOMAIN AT 1.35 ANGSTROM RESOLUTION
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Overview
BACKGROUND: The coxsackievirus and adenovirus receptor (CAR) comprises two, extracellular immunoglobulin domains, a transmembrane helix and a, C-terminal intracellular domain. The amino-terminal immunoglobulin domain, (D1) of CAR is necessary and sufficient for adenovirus binding, whereas, the site of coxsackievirus attachment has not yet been localized. The, normal cellular role of CAR is currently unknown, although CAR was, recently proposed to function as a homophilic cell adhesion molecule., RESULTS: The human CAR D1 domain was bacterially expressed and, crystallized. The structure was solved by molecular replacement using the, structure of CAR D1 bound to the adenovirus type 12 fiber head and refined, to 1.7 A resolution, including individual anisotropic temperature factors., The two CAR D1 structures are virtually identical, apart from the BC, C"D, and FG loops that are involved both in fiber head binding and, homodimerization in the crystal. Analytical equilibrium, ultracentrifugation shows that a dimer also exists in solution, with a, dissociation constant of 16 microM. CONCLUSIONS: The CAR D1 domain forms, homodimers in the crystal using the same GFCC'C" surface that interacts, with the adenovirus fiber head. The homodimer is very similar to the CD2, D1-CD58 D1 heterodimer. CAR D1 also forms dimers in solution with a, dissociation constant typical of other cell adhesion complexes. These, results are consistent with reports that CAR may function physiologically, as a homophilic cell adhesion molecule in the developing mouse brain., Adenovirus may thus have recruited an existing and conserved interaction, surface of CAR to use for its own cell attachment.
Disease
Known diseases associated with this structure: Adrenocortical tumor, somatic OMIM:[188830], Carney complex, type 1 OMIM:[188830], Myxoma, intracardiac OMIM:[188830], Pigmented adrenocortical disease, primary, 1 OMIM:[188830], Spastic paraplegia-7 OMIM:[602783], Thyroid carcinoma, papillary OMIM:[188830]
About this Structure
1EAJ is a Single protein structure of sequence from Homo sapiens with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Dimeric structure of the coxsackievirus and adenovirus receptor D1 domain at 1.7 A resolution., van Raaij MJ, Chouin E, van der Zandt H, Bergelson JM, Cusack S, Structure. 2000 Nov 15;8(11):1147-55. PMID:11080637
Page seeded by OCA on Mon Nov 12 16:41:39 2007
